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Staining Bacteria
2015-01-13
azim58 - Staining Bacteria Dyes to Stain Bacteria Here are some dyes and some information https://www.dojindo.com/Protocol/Bacterial_cell_staining.pdf Note that DAPI can be used, but I'm not sure if it can be cultured afterward Perhaps mCherry or SYTO BC (stains nucleic acids) could be used. http://answers.yahoo.com/question/index?qid=20080609110528AAHLS7m http://aem.asm.org/content/71/8/4679.full?view=long&pmid=16085863 Perhaps Qdots could be used https://lists.purdue.edu/pipermail/cytometry/2005-June/028972.html thiazole orange may be able to be used http://www.ncbi.nlm.nih.gov/pubmed/16999759 FDG may be used. However, this necessitates cell permeabilization. Note that in this paper they also encapsulate single cells in agarose microbeads 20 to 30 microns in diameter http://www.ncbi.nlm.nih.gov/pubmed/2128011 they do single cell genomic studies in this paper http://www.mendeley.com/research/specific-sorting-single-bacterial-cells-mi crofabricated-fluorescenceactivated-cell-sorting-tyramide-signal-amplificat ion-fluorescence-situ-hybridization/
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