Slain2 chimeric transcript

2015-01-13

azim58 - Slain2 chimeric transcript

original document from shen with the sequence that also shows where the
chimeric transcript is
"F:\kurt\storage\CIM Research Folder\DR\2013\6-23-13\chim
transcript\Slain2\mus Slain2.doc"

Primer for Slain2 at the fusion site
slain_fus
TGGCAATGATTGGCTGAAGCC
21 bp
OligoMaster Tm: 54.2

primers last seen at 4-26-13 -20 C chimeric transcript box
I also have some aliquots here: 7-2-13d1700 -20 C box

slainF
CCTGCCCTTAATAGGTTTTCTCC
23bp
55.2 Tm

slainR
CTTTCCAACTGTCATCTTTCATGC
24bp
53.9 Tm

products from slain primer pairs
-sequence PCR amplified by slainF and slainR
--Primer-Blast results for slainF and slainR
-sequence generated from slain fus and pSMART2IF forward screening primer
-sequence generated from slain_fus and reverse pSMART2IF primer
-pSMART2IF_for plus slain_fus
-pSMART2IF_for plus slainR
-slainF plus slain_fus

slain2 chimeric transcript in pSMART2IF vector

Primer order form
"F:\kurt\storage\CIM Research Folder\DR\2013\6-23-13\chim
transcript\Primer order form Kurt 6-23-13.xls"

list of some sequencing results for slain primers portal 7-18-13

azim58wiki: