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Shrimp Alkaline Phosphatase Promega
2015-01-13
azim58 - Shrimp Alkaline Phosphatase Promega Link to information sheet: http://www.oardc.ohio-state.edu/tomato/604.03/Module1/promega%20shrimp%20al kaline%20phosphatase.pdf Promega SAP 1 u/uL (500 u in tube total) Cat # M820A or 25856303. Should be stored at -20 C SAP can inactivated at 65 C 15 min, whereas CAP (Calf Alkaline Phosphotase) cannot be. Main Points from Protocol * 10 units SAP/μg vector in a final volume of 30–50μl. Stock is 1 unit/uL * Incubate at 37°C for 15 minutes. * Incubate at 65 C for 15 min SAP Protocol
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