Home
News
Feed
search engine
by
freefind
advanced
Shortcut for redissolving primers
2015-01-13
azim58 - Shortcut for redissolving primers Just multiply the number of nmol of lyophilized powder by 20 and add this number of uL of H20 or TE (most people use TE) buffer to the primer. Example 17.87 nmol 17.87*20 = 357.4 So add 357.4 uL TE Justification (17.87/1000*umol)/(y/(1*10^6*uL)*L) = 50 uM -> y = 357.4 uL 357.4/17.87 = 20
azim58wiki: