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SfiI Site
2015-01-13
azim58 - SfiI Site Restriction Site GGCCNNNNNGGCC Actual Sequence in PCANTAB plasmid ggcccagccggcc * Unique part of Sequence: cagcc Actual Sequence in scFv MHV_BACK_SfiI primer * GGCCCAGCCGGCC * Unique part o CAGCC Actual Sequence in pTriplEx2 * SfiIA Site o GGCCATTACGGCC o Unique part: ATTAC o Note that there was a discrepancy between the SMART cDNA library and the pTriplEx2 vector sequence information. After contacting Clontech, they responded (12-6-11) and informed me that they think the user manual has the correct sequence. So "ATTAC" is the correct sequence. * SfiIB Site o GGCCATTACGGCC o Unique part: GCCTC
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