Sent selected colony plasmids from 16E3 library for sequencing 8-13-12

2015-01-13

azim58 - Sent selected colony plasmids from 16E3 library for sequencing
8-13-12

Glycerol stocks from the 16E3 library were spread out onto LB plates to
obtain single colonies. 10 colonies from each stock were grown overnight
and miniprepped. The plasmids were used for sequencing, and the remaining
material was stored.

Item: Selected colony Plasmids from 16E3 library 8-13-12 KW at box in -20
C nearest to benches
9 original glycerol stock were spread onto plates. 10 colonies from each
plate (actually only 8 from 29A5 glycerol stock) were miniprepped.
see Tumor vs normal on 2-17-12_16E3 spot 2 for information about the
selection of the spots.

Sequencing results found here
"C:\kurt\storage\CIM Research Folder\DR\2012\8-16-12\sequencing
results\8-15-12 Kurt"

"C:\kurt\storage\CIM Research Folder\DR\2012\8-16-12\sequencing
results\16E3 Pool Sequencing Results.SPF"
"C:\kurt\storage\CIM Research Folder\DR\2012\8-16-12\sequencing
results\Summary of 16E3 Pool Sequencing Results 8.docx"

I would like to analyze these sequences to see if they are in frame, and
to see how soon a stop codon occurs.

Analysis of sequences for stop codons 9-10-12
"C:\kurt\storage\CIM Research Folder\DR\2012\9-10-12\tumor lib
sequences\Analysis of sequences for stop codons 9-10-12.docx"
Sequencher file
"C:\kurt\storage\CIM Research Folder\DR\2012\9-10-12\tumor lib
sequences\Analysis of sequences for stop codons 9-10-12.SPF"

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