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SMC1fs PCR in 4T1 cDNA lysate pools 3-30-12

2015-01-13

azim58 - SMC1fs PCR in 4T1 cDNA lysate pools 3-30-12 experiment performed on 4-2-12 Many tumor cDNA lysate pools/spots other than the SMC1fs positive control spot exhibited binding when anti-SMC1fs sera was applied to the array. We are curious if some of these pools might contain clones with the SMC1fs gene in them. We will do a PCR to test this. Note that there is a possibility that the protein is not SMC1fs, but does have some type of homologous sequence to it. Primers from Shen: SMC1 Mus Jan 18 FW and RV at SMC1 9-20-10 KW 4 C * the sequence: SMC1-mou-RV: GAGCTGTCCTCTCCTTG * >> 54.9 Salt adjusted Tm SMC1-mou-FWD: CTGTCATGGGTTTCCTG The amplified FS transcript is 162bp, Wt is 464bp * SMC1fs PCR Settings 3-30-12.ppt Positive control cDNA 4T1 in mouse cDNA 9/25 at Tumor cDNA Expression Library 1-20-11 KW -20 C Notes from Andrey about PCR * 200 nM primer * 5 uL and 20 uL template * Use Taq or dynazyme Results SMC1fs PCR 3-30-12.svg SMC1fs PCR 3-30-12.pdf
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