Response from Sigma about SIINFEKL dilution 3-14-12

2015-01-13

azim58 - Response from Sigma about SIINFEKL dilution 3-14-12

Subject

S7951, SIINFEKL trifluoroacetate salt

Sigma-Aldrich Technical Service Answer

Scientist's Response Via Email (Jessica)
 03/14/2012 01:21 PM

Hello Kurt,

Thank you for contacting Sigma-Aldrich Technical Service. The proper way
to dilute the peptide will depend on the intended application and
concentration. We test the solubility of the product in 1 mg/mL of water.
At higher concentrations, DMSO may be required. Generally, DMSO is fine
to use as a solvent for cell culture as long as the final DMSO
concentration is less than 0.1%. I suggest that choice a solvent based on
a published reference that most closely matches your intended
application. Furthermore, you should always include a "vehicle control"
in your experiment where you add only solvent to the sample.

I hope that you find this information helpful. If you have further
questions, please reply to this email.

Sincerely,
Jessica
Sigma-Aldrich Technical Service

Your Question By Email
 03/12/2012 11:10 PM

TechServ_Request - United States

Form ID : WF9

TechServ_Number : S7951
TechServ_Name : SIINFEKL trifluoroacetate salt
TechServ_Brand : SIGMA
TechServ_Area : TISS
TechServ_use :
TechServ_About : What is the proper way to dilute this SIINFEKL peptide
powder? In some papers, I see that they dilute the peptide in DMSO.
However, in some other papers, I see that they dilute the peptide in PBS.
In these cases though, I'm not sure if they had a DMSO stock first which
they diluted into PBS. I plan perform a T cell killing assay in which the
target cells are first pulsed with this peptide. Should this peptide be
diluted in DMSO, or will the DMSO harm my tissue culture cells. Thanks
for any help you can offer me!

* Customer_Information **

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