Purification of antibodies with random peptides 6-11-12

2015-01-13

azim58 - Purification of antibodies with random peptides 6-11-12

I will refer to the protocol here: Purifying antibodies using random
peptides on TentaGel Beads in Column Protocol

Some items from experiment
Naive predialysis 6-12-12 at Kurt Array 122109 4 C (this solution passed
through the random peptide tentagel bead column, but was not subjected to
dialysis)
tumor predialysis 6-12-12 at Kurt Array 122109 4 C

Naive random peptide column 6-12-12 KW with 0.02% sodium azide at "random
peptide on tentagel bead 6-4-12 4 C" (the 6 different random peptides on
tentagel beads in this column can be found here: Synthesis of Random
Peptides for Antibody Purification 5-17-12
Tumor random peptide column 6-12-12 KW with 0.02% sodium azide at "random
peptide on tentagel bead 6-4-12 4 C"

Unbound tumor sera 1:100 6-12-12 KW at Kurt array 122109 4C (this is the
part of the sera that passed through the column without binding to the
peptides)
Unbound naive sera 1:100 6-12-12 KW at Kurt array 122109 4C

Tumor sera purified with random peptides 6-13-12 KW with 0.02% sodium
azide at "Kurt array 122109"
(the 6 different random peptides on tentagel beads in this column can be
found here: Synthesis of Random Peptides for Antibody Purification 5-17-12
Naive sera purified with random peptides 6-13-12 KW with 0.02% sodium
azide at "Kurt array 122109"
(the 6 different random peptides on tentagel beads in this column can be
found here: Synthesis of Random Peptides for Antibody Purification 5-17-12

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