Protein Production for printing 8-31-12

2015-01-13

azim58 - Protein Production for printing 8-31-12

Items

* Tumor Proteins 8-31-12 KW (in 384 well plate)
  o see 8-26-12 Plasmids for verification
  o Layout of Plate

1
 2
 3
 4

A
 81
 33
 42

B
 41
 31
 21

C
 11
 4
 71

D
 62
 61
 51
 PUC19

created 2 more plates containing 50 uL of protein solution. One plate is
the reverse of the other for printing purposes. Also e coli lysate was
used instead of PUC19 for the negative control.
Tumor Proteins 8-31-12_2 KW (in 384 well plate)

1
 2
 3
 4

A
 81
 33
 42

B
 41
 31
 21

C
 11
 4
 71

D
 62
 61
 51
 E coli

Tumor Proteins 8-31-12_3 KW (in 384 well plate)

1
 2
 3
 4

A
 E coli
 51
 61

B
 62
 71
 4

C
 11
 21
 31

D
 41
 42
 33
 81

* Soluble proteins (glycerol and EDTA was added) (see 8-26-12 Plasmids
  for verification)
  o Soluble Protein 1 8-31-12
    - from top row to bottom row: 81, 33, 42, 41, 31, 21, 11, 4

o Soluble Protein 2 8-31-12
    - from top row to bottom row: 71, 62, 61, 51, PUC19

* Insoluble proteins
  o Insoluble protein 1 8-31-12
    - from top row to bottom row: 81, 33, 42, 41, 31, 21, 11, 4

o Insoluble protein 2 8-31-12
    - from top row to bottom row: 81, 33, 42, 41, 31, 21, 11, 4

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