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Primers for Constructing scFv

2015-01-13

azim58 - Primers for Constructing scFv LINK_HEAVY 40 bp CAGAGCCACC TCCGCCT GAACCGCC TCCACCT GAGGAGAC 88 Tm 68 % GC LINK_LIGHT 34 bp TTCAGGCGGAGGTGGC'TCTGGCGGTGGCGGAICG 90 Tm 71 % GC edit MHV_BACK_SfiI 19 bp CATATGGCCCAGCCGGCCA 58 basic Tm 52 salt-adjusted Tm 62 base-stacking Tm 68 % GC Tm calculated with http://www.promega.com/techserv/tools/biomath/calc11.htm#melt_results edit MKV_FOR_NotI 18 bp GTTCTTGCGGCCGCCCGT 57 basic Tm 52 salt-adjusted Tm 64 base-stacking Tm 72 % GC edit MLV_FOR_NotI 18 bp GTTCTGCGGCCGCCTTGG 57 basic Tm 52 salt-adjusted Tm 62 base-stacking Tm 72 % GC
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