Primers Used for 1st Construction of scFv Genes

2015-01-13

azim58 - Primers Used for 1st Construction of scFv Genes

NotI Site: GCGGCCGC
SfiI Site: GGCCCAGCCGGCC

see also [Table of primers used for scFv construction] (note that I'm not 100% sure the primers in this table that were used are the same as the primers on this page since I added this note at this time: 12-09-2013d2051)
-"C:\Users\kurtw_000\Documents\kurt\storage\CIM Research Folder\DR\2013\12-9-13\Table of primers used for scFv construction 12-09-2013d2052.xlsx"

MHV_BACK1
 CATATGGCCCAGCCGGCCATGGCCGATGTGAAGCTTCAGGAGTC

MHV_BACK2
 CATATGGCCCAGCCGGCCATGGCCCAGGTGCAGCTGAAGGAGTC

MHV_BACK3
 CATATGGCCCAGCCGGCCATGGCCCAGGTGCAGCTGAAGCAGTC

MHV_BACK4
 CATATGGCCCAGCCGGCCATGGCCCAGGTTACTCTGAAAGAGTC

MHV_BACK5
 CATATGGCCCAGCCGGCCATGGCCGAGGTCCAGCTGCAACAATCT

MHV_BACK6
 CATATGGCCCAGCCGGCCATGGCCGAGGTCCAGCTGCAGCAGTC

MHV_BACK7
 CATATGGCCCAGCCGGCCATGGCCCAGGTCCAACTGCAGCAGCCT

MHV_BACK8
 CATATGGCCCAGCCGGCCATGGCCGAGGTGAAGCTGGTGGAGTC

MHV_BACK9
 CATATGGCCCAGCCGGCCATGGCCGAGGTGAAGCTGGTGGAATC

MHV_BACK10
 CATATGGCCCAGCCGGCCATGGCCGATGTGAACTTGGAAGTGTC

MHV_FOR1
 GCCTCCACCTGCAGAGACAGTGACCAGAGT

MHV_FOR2
 GCCTCCACCTGAGGAGACTGTGAGAGTGGT

MHV_FOR3
 GCCTCCACCTGAGGAGACGGTGACTGAGGT

MHV_FOR4
 GCCTCCACCTGAGGAGACGGTGACCGTGGT

MKV_BACK1
 TCTGGCGGTGGCGGATCGGATGTTTTGATGACCCAAACT

MKV_BACK2
 TCTGGCGGTGGCGGATCGGATATTGTGATGACGCAGGCT

MKV_BACK3
 TCTGGCGGTGGCGGATCGGATATTGTGATAACCCAG

MKV_BACK4
 TCTGGCGGTGGCGGATCGGACATTGTGCTGACCCAATCT

MKV_BACK5
 TCTGGCGGTGGCGGATCGGACATTGTGATGACCCAGTCT

MKV_BACK6
 TCTGGCGGTGGCGGATCGGATATTGTGCTAACTCAGTCT

MKV_BACK7
 TCTGGCGGTGGCGGATCGGATATCCAGATGACACAGACT

MKV_BACK8
 TCTGGCGGTGGCGGATCGGACATCCAGCTGACTCAGTCT

MKV_BACK9
 TCTGGCGGTGGCGGATCGCAAATTGTTCTCACCCAGTCT

MKV_FOR1
 GTTCTTGCGGCCGCCCGTTTCAGCTCCAGCTTG

MKV_FOR2
 GTTCTTGCGGCCGCCCGTTTTATTTCCAGCTTGGT

MKV_FOR3
 GTTCTTGCGGCCGCCCGTTTTATTTCCAACTTTG

MLV_BACK
 CAGGCTGTTGTGACTCAGGAA

MLV_FOR
 GTTCTGCGGCCGCCTTGGGCTGACCTAGGACAGT

MLV_BACK
TCTGGCGGTGGCGGATCGCAGGCTGTTGTGACTCAGGAA

Linker light sequence
TTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCG

Linker heavy sequence
CAGAGCCACCTCCGCCTGAACCGCCTCCACCTGAGGAGAC

MHV_BACK_SfiI: CATATGGCCCAGCCGGCCA

MKV_FOR_NOTI:
GTTCTTGCGGCCGCCCGT

MLV_FOR_NOTI:
GTTCTGCGGCCGCCTTGG

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