Primer-Blast results for slainF and slainR

2015-01-13

azim58 - Primer-Blast results for slainF and slainR

Results from Primer-Blast'
Primer pair 1
Sequence (5'->3') Template strand Length Start Stop Tm GC% Self
complementarity Self 3' complementarity
Forward primer CCTGCCCTTAATAGGTTTTCTCC Plus 23 1012 1034 58.79 47.83 4.00
0.00
Reverse primer CTTTCCAACTGTCATCTTTCATGC Minus 24 1416 1393 58.84 41.67
4.00 2.00
Product length 405
Products on potentially unintended templates
>NM_001113423.1 Mus musculus SLAIN motif family, member 2 (Slain2),
transcript variant 2, mRNA

product length = 721
Forward primer 1 CCTGCCCTTAATAGGTTTTCTCC 23
Template 1320 ....................... 1342

Reverse primer 1 CTTTCCAACTGTCATCTTTCATGC 24
Template 2040 ........................ 2017

>NM_153567.2 Mus musculus SLAIN motif family, member 2 (Slain2),
transcript variant 1, mRNA

product length = 799
Forward primer 1 CCTGCCCTTAATAGGTTTTCTCC 23
Template 1320 ....................... 1342

Reverse primer 1 CTTTCCAACTGTCATCTTTCATGC 24
Template 2118 ........................ 2095

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