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PrimeScript Reverse Transcriptase

2015-01-13

azim58 - PrimeScript Reverse Transcriptase from Takara Product Slip and Protocol "F:\kurt\storage\CIM Research Folder\DR\2013\1-16-13\PrimeScript\PrimeScript Reverse Transcriptase Product Slip.pdf" User Manual "F:\kurt\storage\CIM Research Folder\DR\2013\1-23-13\primescript\UTF-8'en-us'2680Q_DS.v1205Da_2.pdf" What is the minimum amount of RNA that can be used in this reaction? In the In-Fusion SMARTer Directional cDNA Library Construction Kit they use 5-100 ng poly A+ RNA. Note that this polymerase may not be ideal for use with poly T primers with only one or two base pairs to bind to the transcript like the primer in the In-Fusion SMARTer Directional cDNA Library Construction Kit for an example of some cDNA results obtained using those primers see received random hexamer tumor library clones 2-7-13
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