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Preparing Tumor cDNA and anti-SMC1fs Ab slide 5-19-13
2015-01-13
azim58 - Preparing Tumor cDNA and anti-SMC1fs Ab slide 5-19-13 Preparing Tumor cDNA and anti-SMC1fs Ab timeline of mouse immunization timeline of tumor injection timeline of SMC1fs injection "F:\kurt\storage\CIM Research Folder\DR\2013\5-19-13\Preparing Tumor cDNA and anti-SMC1fs Ab.pptx" -content on slide { Information about injection details can be found here http://www.azim58.wikispaces.net/Injection+of+mice+with+tumor+cells+and+SMC 1fs Timelines can be found here L:\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Categorical Records\Biodesign\Immunosignature to Antigen Bridge Project\Tumor cDNA Library\tumor injection\timeline Tumor injection = Injected 7000 4T1 Cells (mice were 42 days old at this point) Genetic Immunization = (1 shot per ear with each shot consisting of 1 ug SMC1 wt+fs/pcMVi-rCOMTT and 1 ug CpG2395) Protein boost = (5 ug SMC1fs fused with GST along with Alum) Peptide boost = (5 ug peptide per mouse) }
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