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Preparing Complete Tissue Culture Media
2015-01-13
azim58 - Preparing Complete Tissue Culture Media Preparing Complete Tissue Culture Media Add 100X penicillin/streptavidin to media. Add 10 mL to 1000 mL media. Add 100 mL FBS (fetal bovine serum) to 1000 mL media for 10% FBS New media last seen in 4 C walk in fridge penicillin/streptavidin last seen in -20 fridge in tissue culture room FBS last seen in -20 fridge in tissue culture room Some media contains sodium pyruvate. According to wikipedia (http://en.wikipedia.org/wiki/Sodium_pyruvate), sodium pyruvate is commonly added to cell culture media as an additional source of energy, but may also have protective effects against hydrogen peroxide.
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