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Preparation of a Linearized Vector by PCR
2015-01-13
azim58 - Preparation of a Linearized Vector by PCR PrimeStar Max polymerase seems to work the best for vector linearization see 11-27-12 Polymerase system test for polymerization for initial test of polymerases for linearization 1 ng of template seems to work just fine see 11-27-12 of notebook for an example =========================================================================== Advantage HD can also be used, but doesn't seem to work as well see "C:\kurt\storage\CIM Research Folder\DR\2012\11-5-12\preparation of a linearized vector by PCR 11-5-12.docx" The In-Fusion SMARTer Directional cDNA Library Construction Kit also has instructions for linearization.
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