Plan for modifying pComb3XSS vector 061312

2015-01-13

azim58 - Plan for modifying pComb3XSS vector 061312

found here:

C:\kurt\storage\CIM Research Folder\DR\2012\6-13-12
description of changes actually made (not just plans) can be found here
"C:\kurt\storage\CIM Research Folder\DR\2012\6-13-12\changes actually
made as of 11-5-12\Modifying pComb3XSS vector.docx"

Here is the sequence of the pcomb after the NotI site has been modified
(the 1st In-fusion modification reaction)
"C:\kurt\storage\CIM Research Folder\DR\2012\7-26-12\pcomb sequence
analysis\pcomb_MN.txt"
This sequence will be pComb3XSSv2 (I will no longer try to add the poly T
site near the LacI) <- Note on 10-2-12
last seen at pComb MN 10-18-12 (MN is for modified NotI) at 9-23-12 KW
-20 C

7-29-12
My first in-fusion reaction to modify the NotI site worked. However, my
2nd in-fusion reaction to add the poly Ts near the Lac site did not work,
and Andrey found that some of the primers were wrong. I'll need to
redesign these primers.
see
"C:\kurt\storage\CIM Research Folder\DR\2012\6-13-12\inserting polyT site
corrected.svg"

Oligo Order
"C:\kurt\storage\CIM Research Folder\DR\2012\7-29-12\pcomb\Oligo Order
Kurt 7-29-12.xls"

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8-28-12
Location of some primers

Stock primers stored in "10-3-11 KW contains some phage stuff -20 C box"
Working primers stored at "4 C box 082510 dot 1020"

===========================================================================
RPTFC
RPTFC2B
RPTFC2
RPTFCB
SAPTFC
SAPTRC

===========================================================================

11-5-12 Correct deletion of stop codon

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