Pierce Silver Stain Protocol

2015-01-13

azim58 - Pierce Silver Stain Protocol

Pierce Silver Stain Protocol
Item 24612
http://www.piercenet.com/instructions/2161478.pdf
Note that this pdf contains more information and a more in-depth protocol
than the protocol below

* Wash gel 2 × 5 minutes in ultrapure water.
  o Make sure to handle the gel with clean gloves or sterile
    equipment to avoid later smudges on the gel

* Fix gel 2 × 15 minutes in 30% ethanol:10% acetic acid solution.
* Wash gel 2 × 5 minutes in 10% ethanol, then 2 × 5 minutes in
  ultrapure water.
* Prepare Sensitizer Working Solution (50μL Sensitizer with 25mL
  water).
* Sensitize gel for 1 minute, then wash 2 × 1 minute with water.
  o Make sure that any silver stain reagents are discarded in an
    appropriate silverstain waste container.

* Prepare Stain Working Solution (0.5mL Enhancer with 25mL Stain).
* Stain gel for 30 minutes.
* Prepare Developer Working Solution (0.5mL Enhancer with 25mL
  Developer).
* Wash gel 2 × 20 seconds with ultrapure water, then develop gel for
  2-3 minutes until bands appear.
* Stop with 5% acetic acid for 10 minutes.

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