PhD Phage Display Cloning System

2015-01-13

azim58 - PhD Phage Display Cloning System

PhD Phage Display Cloning System
http://www.neb.com/nebecomm/products/productE8101.asp
Manual
http://www.neb.com/nebecomm/ManualFiles/manualE8101.pdf

M13KE gIII Cloning Vector
Information for this vector can be found on
http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/dna_sequence
s_maps.asp
Map
http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/maps/M13KE_m
ap.pdf
Sequence
http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/Ge
nBank/M13KE.gbk.txt
Pure Sequence
M13KE Sequence
The M13KE vector contains Acc65I-KpnI and EagI at the insert site.

E-mail From NEB on Dec 9th
E-mail to Kathy about this phage display kit on 1-3-12

How does the position of the peptide insert in the PhD Phage Display
System compare with the position of the scFv in the PCANTAB5E plasmid?
Has anyone else used the M13KE vector for an scFv phage antibody library?
If we change the restriction sites of the vector or insert, how will we
do this?

Some Note from reading phage display cloning manual

azim58wiki: