PCR Screen for 13 validated mouse chimeric transcripts 5-2-13

2015-01-13

azim58 - PCR Screen for 13 validated mouse chimeric transcripts 5-2-13

Screened pools from tumor cDNA library for presence of 13 validated mouse
chimeric transcripts from Hojoon.
Mara started this PCR, and then I came in for the "hot start" and
thermocycle part.
experiment performed on 5-2-13

Original Gel Images found here
S:\Research\Cancer_Eradication\Discovering tumor specific antigens\Tumor
cDNA Library\5-2-13

Annotated Inkscape files here
"F:\kurt\storage\CIM Research Folder\DR\2013\5-7-13\chim transc\chimeric
transcript gels 5-2-13.svg"
"F:\kurt\storage\CIM Research Folder\DR\2013\5-7-13\chim transc\chimeric
transcript gels 2 5-2-13.svg"

Table indicating presence of transcripts in different samples
"F:\kurt\storage\CIM Research Folder\DR\2013\5-7-13\chim transc\Chimeric
transcript screen 5-2-13.xls"
Note that 8 of the 13 validated mouse transcripts (from Hojoon) are
present in at least one of the two tumor cDNA library plasmid pools that
I screened. They originally only detected 4 of these transcripts in 4T1
cDNA previously.

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