PCANTAB Sequences

2015-01-13

azim58 - PCANTAB Sequences

PCANTAB Sequences

PCANTAB5 (4472 bp)
PCANTAB5E (4472 bp)
I think these two sequences are identical. I think these sequences came
from "lablife"
https://www.lablife.org/ll?a=showvecinfo&amp;vectorid=5225

PCANTAB5E KW (4547 bp)
This sequence came from Vector NTI. I'm not sure where it came from
before that.

PCANTAB 5 E GB (4517 bp)

9/9/11: Right now I don't know the true sequence of my plasmid.
If my actual plasmid is more similar to the lablife sequence then cutting
with HindIII and EcoRI should yield 3599-2236 = 1363 bp and 4472-1363 =
3359 bp.
If my actual plasmid is more similar to the Vector NTI sequence then
cutting with HindIII and EcoRI should yield 3674-2236 = 1438 bp and
4547-1438 = 3109 bp.

Information from Tien about PCANTAB Plasmid
Tien Olson thinks that the sequence name should be "PCANTAB 5E". I
checked and it should actually be PCANTAB 5 E.
It was given to her by a post-doc at Duke university. It is no longer
commercially available. She thinks it used to be commercially available
from Pharmicia, and she thinks the "lablife" website sequence should be
correct.
She also thinks that the Duke post-doc put in her "own" insert.

Tien received the pCANTAB 5E plasmid from a Duke postdoc named Barbara
Lipes who was an author on this paper:
An entirely cell-based system to generate single-chain antibodies against
cell surface receptors

"C:\kurt\storage\CIM Research Folder\DR\2013\2-2-13\data_download\An
entirely cell-based system to generate single-chain antibodies against
cell surface receptors.pdf"

More information about the insert that Barbara Lipes put in

After this plasmid was still not behaving as expected, Andrey and I
finally just cut the plasmid into many fragments, cloned the fragments
into pJET, and sequenced these fragments.
Sequences of PCANTAB Fragments cloned into pJET 11-11-11

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