Ordered primers for validation 11-19-12

2015-01-13

azim58 - Ordered primers for validation 11-19-12

Ordered primers for validation 11-19-1

Primer order form
"C:\kurt\storage\CIM Research Folder\DR\2012\11-19-12\sequence
analysis\Kurt Primer Order Form 11-19-12.xls"

received primers 11-26-12
stock of primers last seen Tumor Library 11-29-12 KW -20 C Box
10 uM primers last seen in 4 C Kurt 11-16-12

The location of the primers along the transcript can be found in the ape
files in the subfolders here
C:\kurt\storage\CIM Research Folder\DR\2012\11-25-12\cDNA Sequence
Analysis
Basically the primers amplified sequence from the beginning of the
sequence in the bacteria clone to the end of the sequence. In longer
transcripts which contained a stop codon in the bacteria before the end
of the transcript sequence another reverse primer was used to amplify a
shorter sequence as well.

KW4F
CGCAGAGTACATGGGAAGGATC
KW4R
CAGATAGAACAGCAGACACCTTTATTAC
KW11F
CGCAGAGTAGGTTGTGTTAAGTC
KW11R
ACCAGAGATACAATGAACATATTAAAAAG
KW21F
CGCAGAGTACATGGGATGG
KW21R
ACGCTTGTGTGGCATGATTGG
KW21RS
TCAAAACCATTCTACCTCATTAGC
KW31F
CGCAGAGTACATGGGGTACANG
KW31R
CACCATTATTTACAATAAAGTAAATATACAGTTGG
KW41F
AGTATCCATTGAAAATTTATTATGCATTAG
KW41R
CGCAGAGTACATGGGATTCC
KW42F
GGGCAAGTACACGCGATCATAG
KW42R
ATAAAGCAATTCCAAAGTTTATTAGCC
KW51F
CGCAGAGTACATGGGGGAG
KW51R
GACAATGCCCTAATATGTCTGTGTCC
KW51RS
AATTCTTTGGGGCAAGACACTACATAG

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