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Nested pcr screen scheme for frameshift sequences in plasmid 7-31-13
2015-01-13
azim58 - Nested pcr screen scheme for frameshift sequences in plasmid 7-31-13 I plan to screen the pools with a forward vector primer + reverse transcript primer. Then I will do the nested pcr with the forward transcript primer followed by the fus primer. I plan to screen the cDNA with a forward transcript primer + reverse transcript primer. Then I will do the nested pcr with the forward transcript primer followed by the fus primer. I think I will primarily do these screens with smc and slain pSMART2IF_for plus slainR (1502 bp) slainF plus slain_fus (362 bp) sequence PCR amplified by slainF and slainR (405 bp) pSMART2IF_for plus SMC1-mou-RV (163 bp) smc_fus plus SMC1-mou-FWD (124 bp) Primers for amplifying SMC1fs (162 bp)
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