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Mouse Immunization
2015-01-13
azim58 - Mouse Immunization Mouse Immunization * Good adjuvant for getting a T cell response: Incomplete Freunds o Source: Blattmann * Possible immunization schedule: o Genetic immunization 3 times spaced 3 weeks - protein boost does not boost T cell response much - Source: Blattmann o Genetic immunization day 1, genetic immunization day 3, wait two weeks and then a protein boost o CTL responses peak around 6 days after primary immunization or 4 days after boost immunization (Irvine et al., 1997). Spleens or serum can be harvested 2 to 3 weeks after last immunization. (from Current Protocols in Immunology 20.1.19) * Mice: B6 mice give better CTL responses than Balb/c mice o Source: Blattmann
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