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More PCRs for 7 chimeric transcripts in pools 1-41 6-19-13
2015-01-13
azim58 - More PCRs for 7 chimeric transcripts in pools 1-41 6-19-13 Raw gel images here S:\Research\Cancer_Eradication\Discovering tumor specific antigens\Tumor cDNA Library\6-18-13 Annotated diagram of gels here "F:\kurt\storage\CIM Research Folder\DR\2013\6-19-13\chim transc\annotated chimeric transcript gels 6-19-13.svg" Now I can make a spreadsheet that indicates which of the pools (1-41) contain the chimeric transcripts. This spreadsheet is an updated spreadsheet from the one found here F:\kurt\storage\CIM Research Folder\DR\2013\5-24-13\chimeric transcripts\table of results 5-24-13.xlsx on this page PCR Screen for 7 chimeric transcripts in 41 pools 5-21-13 The new spreadsheet is here "F:\kurt\storage\CIM Research Folder\DR\2013\6-19-13\chim transc\table of results 6-19-13.xlsx" Now that I see which pools were positive for certain chimeric transcripts, I can see which pools to screen further. "F:\kurt\storage\CIM Research Folder\DR\2013\6-19-13\chim transc\chim transcripts and pools to screen further.svg" ^Note that Shen has some sera against 10339 (Thap2+Tmem), but he does not have sera against the other chimeric transcripts detected so I don't need to relook at all 7 chimeric transcripts as I was planning to do.
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