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Miniprep and sequencing 2-5-13

2015-01-13

azim58 - Miniprep and sequencing 2-5-13 Miniprepped overnight cultures of clones from random hexamer cDNA library for both the tumor and liver control samples. These clones were then sent for sequencing with the forward and reverse screening primers from the In-Fusion SMARTer Directional cDNA Library Construction Kit T1-10 2-5-13 KW stored at random hexamer library 2-5-13 KW -20 C box L1-10 2-5-13 KW stored at random hexamer library 2-5-13 KW -20 C box P1 2-4-13 KW stored at random hexamer library 2-5-13 KW -20 C box This sample is an ethanol precipitated in-fusion product of the pUC19 linearized vector in-fused with the control insert from the In-Fusion SMARTer Directional cDNA Library Construction Kit. The precipitated pellet was diluted with 20 uL water. See Plan for transformation condition tests 2-4-13
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