Map of Experiments for CTL Flow Cytometry Assay

2015-01-13

azim58 - Map of Experiments for CTL Flow Cytometry Assay

* Gel of OVA plasmid 6-13-11
* Initial 3T3 cell culture 6-20-11
* Test of caspase kit 10-12-11
* Protein boost with OVA 10-13-11
* Flow cytometry 10-13-11
* Caspase kit test (2nd time) 10-17-11
*

* Fresh media for targets and effectors 10-23-11
  o CTL Assay 1: Killing Assay 10-24-11
  o T cell enrichment 10-26-11
  o Caspase Staining 10-26-11
  o Flow Cytometry to analyze cells

* Ova Immunization ELISA 10-25-11
* NIH 3T3 Apoptosis 11-2-11
  o Camptothecin Test 11-9-11
  o Cell Staining 11-10-11
  o T Cell purification 11-21-11

* IL-2 and Peptide Addition 12-7-11
* Killing Assay with Caspase 12-13-11
* T cell assay maintenance
* Killing Assay with Annexin V 12-21-11
* Passaged 3T3 and CRL2116 cells 1-3-12
* Preparation for T cell purification comparison 1-4-12
* T Cell purification to compare old T cell enrichment kits 1-16-12
  (for 1-5-12)
* peptide preparation 1-23-12
* peptide plate transfer 1-23-12.2216
* Ova protein mouse immunization 1-26-12
* test of decanting after fluor is added 2-3-12
* ELISPOT w/Ova splenocytes 2-9-12
* ELISPOT w/Ova splenocytes 2-11-12
* Imaged ELISPOT plates 2-13-12
* SIINFEKL Peptide Incubation 3-13-12
* ELISPOT w/Ova Peptide 3-14-12
* Ova protein boost 3-30-12
* ova splenocyte test 4-6-12
* killing assay 4-8-12
* bone marrow surgery 6-7-12
* Ova Bullet preparation 6-18-12
* Ova Protein Boost 7-3-12
* Ova ELISA 7-13-12 (Ova ELISA 7-13-12)
* B16F10 Cell Culture 7-17-12
* chromium release assay 8-17-12 to 8-18-12
* CRA with more E:T ratios 8-21-12
* SIINFEKL dilution 9-26-12
* Ova protein boost 10-2-12
* Ova CTL Assay 10-8-12
* Ova mouse immunization 10-15-12
* CRA parameter test 10-18-12
* ova ctl assay w/o tissue culture flask 10-20-12
* CRA Parameter Test 10-18-12
* Ova CTL Assay w/o tissue culture flask 10-20-12
* aged-young cr release assay 1-4-13
* obtained splenocytes from injured mice 1-6-13
* aged-young cr release assay 1-10-13
*

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