Letter to companies about phage display vector

2015-01-13

azim58 - Letter to companies about phage display vector

Hi _ representative,

I need to construct a phage antibody library, and I am wondering if you
sell a plasmid that is suitable for this. The plasmid would need to be
similar to the plasmids used in some phage library papers. For example,
one such plasmid is the Phen1 plasmid. I need the following features.

1. The plasmid has SfiI and NotI sites for cloning my gene. My gene will
be a scFv (single chain fragment variable) gene.
2. The inserted gene will be expressed as a fusion with the p3 phage
protein so that the gene can be displayed on the phage (I have an M13
based phage in mind).
3. The plasmid has an f1 ori or an M13 ori or another origin of
replication compatible with incorporating the plasmid into phage.

Do you sell any plasmid that would work for these requirements? Thanks
for any help you can offer me.

Best regards,
Kurt Whittemore

Graduate Student
Arizona State University
BIODESIGN INSTITUTE
Center for Innovations in Medicine
1001 S McALLISTER AVE
TEMPE, AZ 85287

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