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Isolating Phage from Bacteria
2015-01-13
azim58 - Isolating Phage from Bacteria After I transform my bacteria, how will I isolate the produced phage? Here's some information on the topic. Infection and growth from single colony Single bacterial colonies obtained following a procedure similar to that described for titration above were picked and grown overnight in 2XTY-ampicillin-chloramphenicol at 30 C, 250 r.p.m. For each phage production protocol, after overnight growth phagemids were collected from the supernatant by centrifugation (4000 r.p.m., 30 min), and the levels of phage production determined by titration in DH5aF cells plated on both 2XTYampicillin and 2XTY-chloramphenicol plates. From Eliminating Helper Phage from Phage Display paper
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