Instructions for constructing Figure 2b heatmap 10-28-12

2015-01-13

azim58 - Instructions for constructing Figure 2b heatmap 10-28-12

Obtain naive gpr files

S:\Research\Cancer_Eradication\Users\kwhittem\kwhittem\kwhittem\Records
in CIM Folder\Dated Records\12-21-09 Unpurified Rabbit SMC1 on
array\12-21-09 array files
Obtain smc1 gpr files

S:\Research\Cancer_Eradication\Users\kwhittem\kwhittem\Raw Data Often
Originally on Research Drive\3-18-10\results

Put these gpr files in a new folder and drag into Genespring. Create a
new experiment without any normalizations. Alternatively, use the
Genespring experiment already created.

File->Open Genome or Array->data->Alta 10K

Select the sorted random peptide list

Gene Lists->Kurt->SMC1 temp Analysis with Phil on 5-6-10->108 peptides
sorted by scores from raw values
Select the experiment: Experiments->Kurt->031810 Blocked by SMC1 20 Steps
2->Default interpretation

Then make the heatmap by going to tools->clustering->condition tree and
use all of the default paramaters

Change the lower color to green and the upper color to red
Change the colorbar range.
High 30000
Normal 3000
Low 0

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