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In-Fusion SMARTer cDNA Library Manual
2015-01-13
azim58 - In-Fusion SMARTer cDNA Library Manual In-Fusion SMARTer cDNA.pdf and here "C:\kurt\storage\CIM Research Folder\DR\2013\3-13-13\In-Fusion SMARTer cDNA.pdf" Sequencing primers Forward Screening Primer (10 μM) TCACACAGGAAACAGCTATGA Tm of 50.3 according to oligomaster spreadsheet Reverse Screening Primer (10 μM) CCTCTTCGCTATTACGCCAGC Tm of 56.2 according to oligomaster spreadsheet The M13 forward and reverse primers can also be used M13 Forward Sequencing Primer (Sense Strand): 5'-GTAAAACGACGGCCAGT-3' M13 Reverse Sequencing Primer (Antisense Strand): 5'-AGCGGATAACAATTTCACACAGGA-3' SMARTer IIA Oligonucleotide Sequence as determined by sequencing AAGCAGTGGTATCAACGCAGAGTACATGGG ------ Other information pSMART2IF Transcript orientation question 6-23-13
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