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IgG Purification with Protein G
2015-01-13
azim58 - IgG Purification with Protein G Protein G Agarose from Thermo Scientific (there are several different versions of this kit) 20397, 20398, 20399, 22851, 22852 Original Kit Instructions http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&a mp;cd=1&ved=0CDYQFjAA&url=http%3A%2F%2Fwww.piercenet.com%2Finstruct ions%2F2160576.pdf&ei=zOIyT6z9CcnciQKZ-KmHCg&usg=AFQjCNHB1cJCcac0kS k72_k0yZGxgdWyMg&sig2=hcLMxdeqfhpqI_ngOrLS8w Pierce Protein G Agarose 2-8-12.pdf Modifications or Additional Notes to Protocol * It may be better to incubate sera/binding buffer solution in column with protein G solution while the column is sealed and shaking on a rocker for a time * Note that when nanodropping, make sure to blank with a solution containing the elution buffer and neutralization buffer in the appropriate ratio * Binding Buffer: 0.1 M Sodium Acetate pH 5.0 (pH adjusted with 1 M HCl) * Elution Buffer: 0.1 M citric Acid pH 2-3
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