Identification of tumour antigens by serological analysis of cDNA expression cloning. paper

2015-01-13

azim58 - Identification of tumour antigens by serological analysis of
cDNA expression cloning. paper

Identification of tumour antigens by serological analysis of cDNA
expression cloning. paper

Li G, Miles A, Line A, Rees RC.

Cancer Immunol Immunother. 2004 Mar;53(3):139-43. Epub 2004 Jan 13.
Review.
2004

School of Science, The Nottingham Trent University, Clifton Lane, NG11
8NS, Nottingham, UK.

article is accessible
"C:\kurt\storage\CIM Research
Folder\DR\2013\1-26-13\some_serex_papers\Identification of tumour
antigens by serological analysis of cDNA expression cloning..pdf"

Notes
Identification of tumour antigens by serological analysis of cDNA
expression cloning
Cancer Immunology Immunotherapy
2004
Greece

q
The immunoscreening of cDNA expression
libraries constructed from human tumour tissues with
antibodies in sera from cancer patents (SEREX:
serological identification of antigens by recombinant
expression cloning) provides a powerful approach to
identify immunogenic tumour antigens

they start the paper off by talking about CTL, MHC, and peptides

q
It would be surprising
if cancer antigens induced only a cellular
response and no antibodies. Furthermore, the development
of high-titre IgG requires CD4 T-cell help.

SEREX
a novel strategy using the antibody
repertoire of cancer patients for the molecular definition
of antigens was developed by Pfreundschuh and his
colleagues Sahin and Tu¨ reci [15]
Human neoplasms elicit multiple specific immune responses in the
autologous host. paper

Their description of SEREX uses phage to present the tumor peptides

table 1 has the antigen categories identified

q
antigen overexpression can lead to immunogenicity as it
does in the case of HER-2/neu [36].

q
aetiologically
relevant gene products

^?definition of this?
of or relating to the physical study of causation

q
We have developed an array technique which can be
used to rapidly analyse humoral response of multiple
SEREX antigens using allogeneic sera.

q
antibodies in
human sera that react with bacterial or phage components
can be eliminated by preabsorption of the sera
with E. coli–phage lysate

they preabsorb antibodies against IgG as well
q
eukaryotic expression systems for SEREX
should be developed

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