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Ideal results of ds cDNA Synthesis
2015-01-13
azim58 - Ideal results of ds cDNA Synthesis Basically a moderate strong smear from 0.5 to 5 kb should be detected. A good overview of what a 2nd strand synthesis looks like can be found in the SMARTer cDNA library construction kit manual In-Fusion SMARTer Directional cDNA Library Construction Kit Here is the relevant page from the manual "C:\kurt\storage\CIM Research Folder\DR\2013\1-28-13\cDNA\Typical results of PCR and ds cDNA Synthesis.pdf"
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