High throughput proteomic strategies for identifying tumour-associated antigens. paper

2015-01-13

azim58 - High throughput proteomic strategies for identifying
tumour-associated antigens. paper

High throughput proteomic strategies for identifying tumour-associated
antigens. paper

Gunawardana CG, Diamandis EP.

Cancer Lett. 2007 Apr 28;249(1):110-9. Epub 2007 Feb 15. Review.
2007
Department of Pathology and Laboratory Medicine, Mount Sinai Hospital,
Toronto, Ont., Canada.

article is accessible
"C:\kurt\storage\CIM Research
Folder\DR\2013\1-26-13\some_serex_papers\High throughput proteomic
strategies for identifying tumour-associated antigens..pdf"

Notes
High throughput proteomic strategies for identifying tumour-associated
antigens

University of Toronto Canada
2007
Cancer Letters Elsevier

Serological analysis of recombinant cDNA expression libraries (SEREX)

q
The development of SEREX [8] in the 90s offered
a high-throughput approach to analyze the humoral
response against TAAs in cancer patients.

pros
avoid artifacts of cultured cells

con
antibodies to non-tumor associated antibodies

q
A database
(http://www2.licr.org/CancerImmunomeDB/), Cancer
Immunome Database, contains all the autoantigens
identified by SEREX.

===========================================================================
SERPA
2D western blot -> mass spec

pro
fast
post-translational modifications

con
limited material
co-migrating proteins
issues with reproducibility

===========================================================================
protein microarrays

q
‘‘immunome’’ of a cancer

biased approach (with known analytes) (autoantibody or autoantigen)

Invitrogen protoArray prospector

unbiased approach (new analytes can be discovered) (phage display or cell
lysate)

Invented in 1985 by Smith [21], phage display is a
powerful proteomic tool used to express proteins or
domains of proteins.

pro
high throughput
a lot of data

con
cost

pattern recognition software

q
The Luminex xMAP technology
appears to be very promising as 100 or more
analytes can be studied in one sample alone.

^this is an automated ELISA system

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