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Freezing Tissue Culture Mammalian Cells
2015-01-13
azim58 - Freezing Tissue Culture Mammalian Cells Pellet the cells and resuspend in 1 mL freezing media in a cryogenic vial. Place the vial into the cryogenic container containing isopropanol (such a container allows the temperature to drop slowly) and place this container into -80 C overnight. Transfer vial to -150 C for long term storage. =========================================================================== Cryogenic vials last seen in tissue culture room in 2nd or 3rd drawer in dresser near door. Tissue culture freezing media (some amount of DMSO and FBS?)
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