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Flow Cytometry CTL Protocol

2015-01-13

azim58 - Flow Cytometry CTL Protocol Overall flow cytometry CTL assay diagram Flow Cytometry CTL Protocol * Mouse Immunization * Surgical Removal of Spleens * Splenocyte Preparation Protocol * Induction of Cytolytic Activity in CTL Precursors * Purification of T cells with Robosep Kit * Killing Assay * Killing Cells with Camptothecin for Positive Control * Caspase Kit * Annexin V protocol * Annexin V and PI or 7-AAD protocol * 7-AAD or PI protocol * Flow Cytometry Note that I may want to: make shortcut sheet of calculations for CTL assay Older Information
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