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Dynazyme Protocol
2015-01-13
azim58 - Dynazyme Protocol extension time for dynazyme: This is from the excel spreadsheet attached below. The amount of primers reflect the amount for a primer concentration of 20uM. If the primer is a different concentration other than 20uM, change the volumes or dilute primer down to said concentration. Protocol Here "C:\kurt\storage\CIM Research Folder\DR\2013\2-26-13\dynazyme\pcr_protocol\Dynazyme PCR.xls" In our lab we use Dynazyme II DNA polymerase I have some aliquots of dynazyme here: 7-2-13d1700 -20 C box official manual here http://www.thermoscientificbio.com/uploadedFiles/Resources/f503sl_dynazyme2_prodinfo_A4_TS_v1_low.pdf and here "F:\kurt\storage\CIM Research Folder\DR\2013\2-26-13\dynazyme\dynazyme II dna polymerase product information.pdf" see also Thermocycle Conditions portal 5-9-13 see also Dynazyme Colony PCR Protocol see also comparison of primestar max with dynazyme 7-3-13
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