Do I have glycerol stock of A B or C ligation 5-21-13

2015-01-13

azim58 - Do I have glycerol stock of A B or C ligation 5-21-13

Do I have glycerol stock of A, B, or C ligation?

So far this issue is unresolved.

Another related question is whether I still have DNA of the A, B, or C
ligation so that I can do another transformation and then make a glycerol
stock.

Here are some notes investigating this issue
<investigation>
{
Need to find glycerol stock for entire tumor cDNA library (if I even made
such a stock.

This stock would have been made before 1-8-12
Actually, I may not have such a glycerol stock
Sequencing of tumor B & C Ligations 1-18-12
I don't have any record of making a total glycerol stock of the B and C
tumor library ligations. I made overnight cultures from colonies to
determine the complexity of these libraries on 1-11-12. I may just need
to do another transformation.
What aboutthe A ligation? Do I have a glycerol stock for this one?
Found some glycerol stock in -80 C "Kurt's glycerol stock" box 3-11-11
cim items
Do I have glycerol stock of A B or C ligation 5-21-13
}
</investigation>

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