Diversity of HIV-1 Vpr interactions involves usage of the WXXF motif of host cell proteins.

2015-01-13

azim58 - Diversity of HIV-1 Vpr interactions involves usage of the WXXF
motif of host cell proteins.

Diversity of HIV-1 Vpr Interactions Involves Usage of the WXXF Motif of
Host Cell Proteins

BouHamdan, M., Xue, Y., Baudat, Y., Hu, B., Sire, J., Pomerantz, R.J.,
Duan, L.-X., 1998. Diversity of HIV-1 Vpr interactions involves usage of
the WXXF motif of host cell proteins. Journal of Biological Chemistry
273, 8009-8016.

location
/home/kurt/kurt/storage/laptop_L_DR/2012/11-4-12/110412d1024/Diversity of
HIV-1 Vpr Interactions Involves Usage of the WXXF Motif of Host Cell
Proteins.pdf

Diversity of HIV-1 Vpr Interactions Involves Usage of the WXXF Motif of
Host Cell Proteins
notes
11-4-12

(Received for publication, October 9, 1997, and in revised form, December
9, 1997)
Mohamad BouHamdan‡§, YanNing Xue‡§, Yves Baudat¶, Baocheng Hu‡,
Josephine Sire¶, Roger
J. Pomerantz‡, and Ling-Xun Duan‡i
From the ‡The Dorrance H. Hamilton Laboratories, Center for Human
Virology, Division of Infectious Diseases,
Department of Medicine, Jefferson Medical College, Thomas Jefferson
University, Philadelphia, Pennsylvania 19107 and
¶INSERM U372, Pathogenie des infections a lentivirus, BP 178, 13276
Marseille-Cedex 9, France

long term goal would be to make an anti-viral agent to interfere with
HIV-1 replication

q
Applying this target protein docking model
for further molecular analyses is now possible due to the development
of the phage display system

Vpr protein very important for HIV virus

q
We demonstrate that most of the peptides that
bind to Vpr contain a common motif, WXXF.

q
A phage-display peptide library
kit (New England Biolabs, Beverly, MA)

transfection protocol

? I don't know the purpose of the CAT assays. Probably will become clear
later.

His-deficient medium.
? don't know the significance of His deficient medium

q
These results clearly indicate that the
amino acids Trp (222) and Phe (225) of UDG, corresponding to
the WXXF motif of UDG, are necessary for binding to Vpr.

they fused WXXF to the CAT gene
CAT is a foreign protein to HIV-1

Growth in the absence of histidine is an indication of the interaction
between hybrid proteins.
(in a yeast two hybrid system I guess)

UDG contains WXXF

q
This
study illustrates a potential new strategy in the targeting of
anti-viral agents into virions that may permit a feasible avenue
to interfere with HIV-1 replication in vivo.

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