Details of scFv Construction 7-7-10

2015-01-13

azim58 - Details of scFv Construction 7-7-10

Details of scFv Construction 7-7-10
Primer Sequences
[[../5-27-10 Records/Map of Phage Library Box 5-27-10.ods|Location of
Tien's phage info:
smb:peptide.biodesign.asu.edu/research/CIM/Mol_Biol_Tech/Phage%20display]]
[[smb:peptide.biodesign.asu.edu/research/CIM/Mol_Biol_Tech/Phage
display/phage display library/primer order/Invitrogen_ Oligo
Order_042209.xls|Location of Primer Order Form:
smb://peptide.biodesign.asu.edu/research/CIM/Mol_Biol_Tech/Phage%20display/
phage%20display%20library/primer%20order/Invitrogen_ Oligo
Order_042209.xls]]
Source of Primer Sequences: [[../5-25-10 Records/Phage Library
Planning/Optimization of primer sequences for mouse scFv repertoire
display library construction.pdf|Optimization of primer sequences for
mouse scFv repertoire display library construction]]
Note that the first part of the sequences do not correspond to the
sequences of the primers that Tien ordered because the first part of
their sequences matched with the pHEN1 vector whereas we are using the
pCANTAB5 vector. The last part of the sequence matches though.

What are the sequences of the mouse immunoglobulin genes?
10-26-10
The Zhou paper constructed their primers to match the sequences in the
Kabat et al database. Specifically they used the sequences from Sequences
of Proteins of Immunological Interest 4th edition 1987 pg. 508-553.
I have found several sources of antibody sequences.
THE INTERNATIONAL IMMUNOGENETICS INFORMATION SYSTEM
http://imgt.cines.fr/
http://www.bioinf.org.uk/abs/
NCBI IgBlast
http://www.ncbi.nlm.nih.gov/igblast/showGermline.cgi
http://www.kabatdatabase.com/index.html
I may want to look at the following paper: Accessing the Kabat antibody
sequence database by computer
I checked out the following book from the library: Sequences of Proteins
of Immunological Interest 5th edition 1991

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