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DNA gel extraction
2015-01-13
azim58 - DNA gel extraction Gel extraction results seem to improve when DNA is extracted immediately -evidence? --DNA fragments from a screen for a slain transcript were gel extracted immediately and the DNA sequencing results seemed to have good quality (sequencing results from slain screen 7-28-13), whereas a potential SMC1fs fragment was not extracted immediately and these sequencing results were not as good (sequencing results 7-22-13). Does it make a difference to extract DNA immediately? Does it make a difference to mix sample by vortex vs inversion?
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