DNA Sequencing

2015-01-13

azim58 - DNA Sequencing

Next generation sequencing is basically high-throughput sequencing that
uses technologies that parallelize the sequencing process, producing
thousands or millions of sequences at once. High-throughput sequencing
technologies are intended to lower the cost of DNA sequencing beyond what
is possible with standard dye-terminator methods.
There are many different new methods being explored

Deep Sequencing
http://en.wikipedia.org/wiki/Deep_sequencing
Depth in DNA sequencing refers to the number of times a nucleotide is
read during the sequencing process. Deep sequencing indicates that the
coverage, or depth, of the process is many times larger than the length
of the sequence under study.
The term "deep" has been used for a wide range of depths (>7x),[citation
needed] and the newer term "ultra-deep" has appeared in the scientific
literature to refer to even higher coverage (>100x).

RNAseq is deep sequencing of the transcriptome
Deep sequencing of transcriptome, also known as RNAseq, provides both the
sequence and frequency of RNA molecules that are present at any
particular time in a specific cell type, tissue or organ. Counting the
number of mRNAs that are encoded by individual genes provides an
indicator of protein-coding potential, a major contributor to phenotype

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