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Cut to test for ligation 10-15-12

2015-01-13

azim58 - Cut to test for ligation 10-15-12 Cut to test for ligation 10-15-12 Numbers associated with pcomb rcomp ligation 10-26-12 So I will be able to see if the ligation worked or not, and whether or not my plasmid is the way that I expect it is. None of the pcomb rComp ligation plasmid samples even had any DNA on the gel! Anyway, regardless of these results, the next time I do a digest and ligation I will need to make sure that the uncut plasmid is well separated from the cut plasmid backbone.
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