Chromium Release CTL Assay with Ova 8-21-12

2015-01-13

azim58 - Chromium Release CTL Assay with Ova 8-21-12

I repeated the experiment from before using the intended E:T ratios 100,
30, 10, 3 (I made a pipetting error during the last experiment which
prevented me from using these ratios). During this experiment I thought I
had enough effectors left over from before, but many of them had died off
over several days. I had to obtain the spleen from another Ova mouse, and
this delayed the amount of time I planned to pulse my target cells (about
5 hr instead of 2 hr). I hope that the target cells survived this
treatment and retained the peptide. I will find out the results soon.

Item: Ova Mouse sera 8-21-12 KW at CTL 6-13-11

Plate Map
"C:\kurt\storage\CIM Research Folder\DR\2012\8-21-12\ctl\ctl plate map
8-21-12.xlsx"

Summary of Results
"C:\kurt\storage\CIM Research Folder\DR\2012\8-22-12\ctl\Summary of Ova
CTL Experiment 8.docx"
Version of file for indexing 8-22-12

10-9-12 The 6 hr plate was read again to see how much the radiation
decreased after more than one month.
Results stored here
"C:\kurt\storage\CIM Research Folder\DR\2012\10-9-12\CTL\Images\recount
of old plate\recount of 8-21-12 ova plate.jpg"
Image of old plate
"C:\kurt\storage\CIM Research Folder\DR\2012\8-22-12\ctl\ova ctl 6 hr
8-22-12.jpg"
The radiation did decrease, but was still very detectable.

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