Home
News
Feed
search engine
by
freefind
advanced
Cell Lysate Print 3
2015-01-13
azim58 - Cell Lysate Print 3 experiment performed on 7-8-11 (codelink condition test) used protocol CodeLink.hrp -CodeLink slides used and aminosilane slides tested -blocked 30 min with CodeLink ethanolamine blocking buffer (0.1 M Tris, 50 mM EtOH Amine Methol" (ethanolamine?), pH 9, blocked 30 min with BSA containing buffer -4 slides 5 nM primary (purified rabbit anti-h-SMC1fs 17 mer) -6 slides 1:500 sera (rabbit anti-h-SMC1fs) -5 nM secondary (Bethyl biotinylated goat anti-Rabbit IgG) -5 nM tertiary (streptavidin AF647) results here F:\kurt\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Dated Records\2011\7-12-11\Cell Lysate Array 3 Results and here "F:\kurt\storage\CIM Research Folder\DR\2013\9-5-13\Summary of Print Runs 9-5-13.svg" lab notebook notes corresponding to this experiment "F:\kurt\storage\CIM Research Folder\DR\2013\9-5-13\lab notes corresponding to cell lysate 3 experiment 7-8-11.pdf" These codelink slides still appeared quite smeared.
azim58wiki: