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Cell Lysate Print 13

2015-01-13

azim58 - Cell Lysate Print 13 Cell Lysate Print 13 (Grace BioLabs Nitrocellulose Slides 16 hr room temperature incubation; one with Super G blocking buffer one without) Slides ran around 1-20-12 Spacing of Spots when printed: 500 um Primary: Balb/Nat SMC1 27 mer Secondary: goat anti mouse Data Location: L:\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Categorical Records\Biodesign\Immunosignature to Antigen Bridge Project\Tumor cDNA Library\Printing Library onto array\Cell Lysate Test Prints\BSA Super G Test 1-21-12 2-7-12 I was going to align and analyze these arrays, but after looking at the images of the slides, I see that the spots were very faint and quite smeared. Did the overnight condition cause these undesirable results? Johnny and I will print 4 more slides for a test. 1 slide BSA primary incubation 1 hr 1 slide Super G primary incubation 1 hr 1 slide BSA primary incubation 16 hr 1 slide Super G primary incubation 16 hr
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