Cell Lysate Print 11

2015-01-13

azim58 - Cell Lysate Print 11

Grace BioLabs Nitrocellulose Slides with Overnight overnight primary
1:500 dilution with low agitation at 23 C
found at 12-13-11 of notebook

"F:\kurt\storage\CIM Research Folder\DR\2013\9-5-13\lab notes
corresponding to cell lysate print 11 experiment 12-13-11 .pdf"

Location of Files
S:\Research\Cancer_Eradication\Users\kwhittem\kwhittem\Raw Data Often
Originally on Research Drive\2011\12-14-11

-graphs
--"S:\Research\Cancer_Eradication\Users\kwhittem\kwhittem\Raw Data Often
Originally on Research Drive\2011\12-14-11\Tecan Power Scanner\SMC1fs
Cell Lysate on Nitrocellulose slide scanned 10 laser power block 1.xls"
--"S:\Research\Cancer_Eradication\Users\kwhittem\kwhittem\Raw Data Often
Originally on Research Drive\2011\12-14-11\Tecan Power Scanner\SMC1fs
Cell Lysate on Nitrocellulose slide scanned 10 laser power block 2.xls"

The 1/100 dilution was detectable above lysate spots with no SMC1fs
protein. However, the 1/1000 dilution was not detectable. I will try
another experiment with a more concentrated primary. I will also test out
the Super G Blocking buffer.

Which scanner is better for Grace Biolabs Nitrocellulose slides?

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