Cell Lysate Plate 9-21-11

2015-01-13

azim58 - Cell Lysate Plate 9-21-11

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Cell Lysate Plate 9-21-11
Wednesday, September 21, 2011
7:10 PM
Denatured proteins: 1% SDS 1 hr 60 C (50 uL original sample)
Plate Layout

1
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 20

A (non-denatured)
 1X
 0.5X
 0.1X
 0.01X
 0.001X
 0.0001X
 0
 GST
 GST-SMC1fs
 Old 1X from 8-10-11
 1X
 0.5X
 0.1X
 0.01X
 0.001X
 0.0001X
 0
 GST
 GST-SMC1fs
 Old 1X from 8-10-11

C (denatured)
 1X
 0.5X
 0.1X
 0.01X
 0.001X
 0.0001X
 0
 GST
 GST-SMC1fs
 Old 1X from 8-10-11
 1X
 0.5X
 0.1X
 0.01X
 0.001X
 0.0001X
 0
 GST
 GST-SMC1fs
 Old 1X from 8-10-11

EDTA was added to samples (final concentration: 1 mM) to prevent
bacterial growth.
GST was obtained from Shen's shelf: GST protein from pGEX2T in PBS-2
1/28/11
SMC1 protein was obtained from Shen's shelf
hSMC1-27mer 1 mg/mL 6/28/11
I diluted this protein 5 fold (40 uL into 200 uL 1X PBS) for 0.2 mg/mL
Nanodrop Concentrations:

Sample
 Concentration (ng/mL)
 260/280

pGEX-SMC1fs Induced
 11.54
 0.82

pGEX-SMC1fs Not Induced
 13.46
 0.73

pET32 TEV
 8.58
 0.55

pET32 TEV Not Induced
 11.25
 0.72

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